$90.00Catalog Number: PYT680-2ugSize: 2ug

VEGF Mouse, His

Vascular Endothelial Growth Factor Mouse Recombinant, His Tag

VEGF Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids (205-324 a.a.) and having a total molecular mass of 16.3kDa.
Mouse VEGF is fused to 20 amino acid His Tag at N-terminus and purified by proprietary chromatographic techniques.

Accession

Q00731

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R.

Biological Activity

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5ng/ml, corresponding to a Specific Activity of 666,000 -2,000,000 IU/mg.

Formulation

The Mouse VEGF contains 20mM Tris-HCl buffer (pH8.0) and 10% glycerol.

Physical Appearance

Sterile Filtered colorless solution.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Source

Escherichia Coli.

Stability

Mouse VEGF although stable 4oC for 4 weeks, should be stored desiccated below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, Vegf120, Vegf164, Vegf188, Vegfa.

Quantity

$90.00

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