Vascular Endothelial Growth Factor (121 a.a.) Human Recombinant, Sf9
Vascular Endothelial Growth Factor-121 Human Recombinant produced in insect cells as an 18kDa homodimer, is a glycosylated, polypeptide chain containing 121 amino acids and having a molecular mass of approximately 36kDa.
VEGF121 circulates more freely than other VEGF forms, which bind more tightly with vascular heparin sulfates.
The VEGF-121 is purified by proprietary chromatographic techniques.
Accession
Biological Activity
Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 2-5ng/ml corresponding to a Specific Activity of 200,000-500,000IU/mg.
Formulation
The protein was lyophilized from a concentrated (1mg/ml) solution with no additives.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Purity
Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Solubility
The lyophilized Vascular Endothelial Growth Factor -121 is soluble in water and most aqueous buffers. The lyophilized VEGF-121 should be reconstituted in PBS or medium containing at least 0.1% HSA or BSA to a concentration not lower than 50ug/ml.
Source
Stability
Lyophilized Vascular Endothelial Growth Factor 121 although stable at room temperature for 3 weeks, should be stored desiccated below
-18oC. Upon reconstitution VEGF-121 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Synonyms
Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.
