Request QuoteCatalog Number: xP374403AVLSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP374403AVLYeast1mgQuote
EP374403AVLE. coli1mgQuote
BP374403AVLBaculovirus200ugQuote
MP374403AVLMammalian Cell200ugQuote

Protein Information

SpeciesAzoarcus sp. (strain BH72)
UniProt IDA1K9L8
Gene NamevalS; Locus:azo2907
Protein NameValine--tRNA ligase
Region Expressed1-998
Expression Tag6xHis
Purity>90%
AA SequenceMELAKSFEPAAIEARRYPEWESRGYFDAGLDTSNPNAFCILLPPPNVTGTLHMGHGFNQT IMDALTRYHRMRGDNTLWQPGTDHAGIATQIVVERQLDAQGVSRHDLGREKFLEKVWEWK EYSGGTITRQMRRLGTSPDWKRERFTMDEGLSKTVTETFVRLYNEGLIYRGKRLVNWDPK LGTAVSDLEVVSEEEDGKLYHILYPFSDGPVGDLRGLTVATTRPETLLGDVAVMVHPEDE RYAHLIGKTVALPLTGRHIPIIADDYVDREFGTGCVKVTPAHDFNDYAVGQRHQLDMIVV LKLDGSVPAVAERYTTDGQPREGVAMPAGVAGLDRVPARDKVVAELEALGLMLEIKAHKL QVPRGDRTNVVIEPMLTDQWFVAMSKPGADGKSITAKALEVVASGEIKFYPENWVNTYNQ WLNNIQDWCISRQLWWGHRIPAWYDDEGRIYVAANEEAALHAWKADLEAEIARLQGEVQS RQSQGQTAEQYPDLAERLSVLHARYEEGTLRQEEDVLDTWYSSALWPFSTLDWTPEWPQK SNPALDLYLPSTVLVTGFDIIFFWVARMVMMTKHITGKIPFKHVYVHGLIRDAEGQKMSK SKGNVLDPIDLIDGIGIDELVQKRTFGLMNPKQAQSIEKKTRKEFPEGIPAFGTDALRFT FASLASPGRDIKFDLARCEGYRNFCNKLWNATRFVLMNCEGQDCGMDPHEPGTCVPGGYL DFSFADRWIVSRLQRTEAEVAAQFEAYRFDLVARAVYEFVWDEYCDWYLELAKVQIQTGT PEQQRATRRTLLRVLETVLRLAHPLIPFITEELWETVAPLAGRKDADSIMLARYPQADMG RIDEASEAQVAELKALIYACRNLRGEMNISPAQRLPLVAAGNAELLGRYAPYLAGLAKLS EVEIVAEIGADELAPVAVAGETRLMLKVEIDIAAERERLTKEIARLEGEVAKAEGKLGNA SFVDRAPAAVVQQERDRLAGFKATLEQLRPQLAKLAGR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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