Request QuoteCatalog Number: xP495152FLKSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP495152FLKYeast1mgQuote
EP495152FLKE. coli1mgQuote
BP495152FLKBaculovirus200ugQuote
MP495152FLKMammalian Cell200ugQuote

Protein Information

SpeciesStaphylococcus carnosus (strain TM300)
UniProt IDB9DND4
Gene NamevalS; Locus:Sca_1269
Protein NameValine--tRNA ligase
Region Expressed1-876
Expression Tag6xHis
Purity>90%
AA SequenceMEMKPKYNPTEVEAGRYQEWVEKGYFRPSGDKSKETYTIVIPPPNVTGKLHLGHAWDTTL QDILTRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGISRHDIGREKFLEEVWSWKD EYASFIRQQWAKLGLGLDYDRERFTLDEGLSKAVRKVFVDMYNKGLIYRGERIINWDPEA RTALSDIEVVHEDVEGKFYHFKYPYADGEGYIEIATTRPETMLGDTAIVVNPDDERYKDV IGKKVILPIVNRELPILADEYVDVEFGSGAMKVTPAHDPNDFEIGQRHNLESIIVMDEEG KMNDKAGKYEGMDRFECRAQLVEDLKEQDLVIKIEDHVHAVGHSERTGAVVEPYLSTQWF VNMEPLAKQALDNQKTDNRINFVPERFEHTFNQWMENIRDWTISRQLWWGHQIPAWYHKE TGEIYVGETEPEDAENWVQDEDVLDTWFSSGLWPFSTLGWPDVESEDYQRYYPTNALVTG YDIIFFWVARMIFQGLEFTGERPFDDVLLHGLVRAEDGRKMSKSLGNGVDPMDVINEYGA DSLRYFLATGSSPGHDLRYSTEKVESVWNFINKIWNAARFSIMNIGEEFKFEDIDLSKNL SLADKWILTRLNETIKTVTDLSDRYEFGEVGRALYNFIWDEFCDWYIEMSKIPMNGEDEA QKQVTRSVLSYVLERTMRMLHPYMPFVTEEIWQNLPHVGETIVTSAWPEVEEAYMFEESK QAMQYVVEIIKAVRQARSEVNTPLSKAIPIYIKTKDAEIKQMLDENQHYLERFGHPSELV ISTDIETPDKAMTSVVGAGEVILPLEGLIDMDKEIARLEKEIDKWQSELDRVDKKLSNEN FVNKAPEKIINEEKAKKHDYQEKFDSVKARIEQLKA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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