Request QuoteCatalog Number: xP457222AXISize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP457222AXIYeast1mgQuote
EP457222AXIE. coli1mgQuote
BP457222AXIBaculovirus200ugQuote
MP457222AXIMammalian Cell200ugQuote

Protein Information

SpeciesActinobacillus pleuropneumoniae serotype 7 (strain AP76)
UniProt IDB3GYI3
Gene NamevalS; Locus:APP7_1562
Protein Name
Region Expressed1-954
Expression Tag6xHis
Purity>90%
AA SequenceMTQNLQMADRFDSSAVEQALYKHWEEQGYFKPTENPSLPSYCIAIPPPNVTGSLHMGHAF QQTLMDTLIRFNRMEGNNTLWQTGTDHAGIATQMVVERKIAAEEGKTRHDYGREAFINKI WDWKAYSGGTISQQMRRLGNSIDWDREHFTMDEGLSNAVKEVFVRLHEEGLIYRGKRLVN WDPKLHTAISDLEVENKESKGSLWHFRYPLANGAKTADGKDYLVVATTRPETVLGDTAVA VHPEDERYQSLIGKTVVLPLANREIPIVADEYVDREFGTGVVKITPAHDFNDYEVGKRHG LPMVNVMTMNADIRAEAEIIGTDGKPLTTYEAKIPADYQGLERFAARKKVVADFEALGLL DEIKPHDLKVPYGDRGGVPIEPMLTDQWYVSVKPLAEVATKAVEDGEIQFVPKQYENLYF SWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVARSEEEVRQKHNLPADLALRQDEDVLD TWFSSGLWTFSTLGWPEQTKELKMFHPTDVLITGFDIIFFWVARMIMFTMHFVKDENGKP QVPFKTVYVTGLIRDEQGQKMSKSKGNVLDPIDMIDGISLEDLLEKRTGNMMQPQLAEKI AKATRKEFENGIAAHGTDALRFTLAALASNGRDINWDMKRLEGYRNFCNKLWNASRFVLT NDKLDLSAGEVEYSLADRWIESKFNRTVGEFREALSQYRFDLAANAIYDFTWNEFCDWYL ELTKPVFANGTEAQKRGASQTLVRVLEKLLRLAHPIMPFITEEIWQKVKGFAGIDADTIM LQPFPKVVKSELDESAEMQIGWIKELIIAVRNIRAESNIAPSKGLEFLVRNVSDEQRKIL AENDRLLKAMAKLDSVQVLSADENAPLSVAKLVGNVEVLIPMAGFINKEAELARLTKEIE KMRGEITRIENKLGNEAFVAKAPEAVIAKEREKMQEYQNGLEKLQTQYQAIENL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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