Request QuoteCatalog Number: xP424769RIMSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP424769RIMYeast1mgQuote
EP424769RIME. coli1mgQuote
BP424769RIMBaculovirus200ugQuote
MP424769RIMMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia bellii (strain OSU 85-389)
UniProt IDA8GVS7
Gene NamevalS; Locus:A1I_02925
Protein NameValine--tRNA ligase
Region Expressed1-812
Expression Tag6xHis
Purity>90%
AA SequenceMKEFPKNYNFIESEKKWQKIWQEKQIYAYDENIAKDETFVVDTPPPTVSGQLHIGHVYSY TQTDFIVRFQRMMGKNIFYPMGFDDNGLPTERLVEKQRQVKAYNMGREELINICNEVVAS EEEKFRSLFNQIALSVDWNLEYQTISPLSRKISQMSFLDLVKKGEVYRNNQPILWDPVDG TALAQADIEDKEKTSFMNYITFKTEANDEFTIATTRPELLPACVAVFYHPDDKRYQHLAG KFAVTPLFNVKVPLLADPLVQQDKGTGLVMCCTFGDQTDITWWKTHNLPLNTIITKKGTI DFPHEIGIDGLKIKEARAKIIDILKEQELFVKQEEITQTVKCAERSGAPLEVLTVPQWFV KTISHKDELLKRANELNWHPKNMKIRLDNWINAISWDWCISRQRYFGVPFPVWYSKRIGE EGKILYADISQLPVDPLKDLPIGYSKYEVEPDLDVMDTWATSSVSPQLSTWGISDEFAVN KDRHGKLFPMDLRPQAHEIIRTWAFYTILKAHLHQNTLPWKNIMVSGWCLAEDRSKMSKS KGNVLVPEKLLEQYGSDVIRYWSANSKLGADTAYSEDVMKNGKRLVNKLWNAAKFVSQHF DKLSDEDKKTNLIDVKEKITHEFDQWIINKLVELVNNATNELQNYEYANAMHLTEKFFWS VFCDNYLEISKTRAYDEENKNPSGQYSSVLTLYHVMQTLLKLFAPFMPHITEELYQILYS ENSIHIKGNWINYGNLNYKIDAKQPERLLEILDHVRKFKAEKNLSIKAEVQLLEVSGIEL SKELTSDLKNVTSAKEVKFKPTNDEIKVSILT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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