Request QuoteCatalog Number: xP421646RNCSize: 0.2-1mg

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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP421646RNCYeast1mgQuote
EP421646RNCE. coli1mgQuote
BP421646RNCBaculovirus200ugQuote
MP421646RNCMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia rickettsii (strain Sheila Smith)
UniProt IDA8GTB4
Gene NamevalS; Locus:A1G_05850
Protein NameValine--tRNA ligase
Region Expressed1-812
Expression Tag6xHis
Purity>90%
AA SequenceMKEFPKNYNFTENEKKWQQIWQEKQIYAYNPNVAKEETYVIDTPPPTVSGQLHIGHVYSY TQTDFIVRFQRMMGKNIFYPMGFDDNGLPTERLVEKQKQIKAYNMERSEFIKICEEVVES EEEKFRSLFNQIALSVDWSLEYQTISPLSRKISQMSFLDLVQKEEIYRTNQPILWDPVDG TALAQADIEDKEQTSFMNYITFKTEQGDPLTIATTRPELLPACVAVFYHPDDGRYKHLAG KSAITPLFNEQVPLLADPLVRQDKGTGLVMCCTFGDQTDITWWKTHNLPLKTIITKKGTI DCQHETSIDGLKIKEARTKIIAILKEQELLIKQEDITHTVKCAERSGAPLEILTVPQWFV KTISHKEELLKRANELNWHPKNMKIRLENWINAISWDWCISRQRYFGVPFPVWYSKRVGE EGKILYADITQLPIDPLKDLPMGYSKEEVEPDYDVMDTWATSSVSPQLSTHGISDDFAVN KDRHDKLFPMDLRPQAHEIIRTWAFYTILKAHLHQNTLPWKNIMISGWCLAEDRSKMSKS KGNVLVPEKLLEQYGSDVIRYWSANSKLGADTAYSEDVMKNGKRLVNKLWSAAKFVFIHF DKLKGEDKKASLLDIKEKITNEFDKWMVNKLVELVKLATNELQNYEYANAMHLTEKFFWV VFCDNYLEISKTRSYDEEHKNPQGQYSSILTLYHVMQTLLKLFAPFMPHITEELYQILYS ENSIHVKGSWVNYSDLNYEINAKGAEGLLEILDIVRKFKAEKNLSIKAPIKLLEVSGIVL SAELAEDLKNVTSADEIQFEMKDDKIKVNIIL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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