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Recombinant Valine--tRNA ligase (valS)

Recombinant Valine--tRNA ligase (valS) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP426974STCYeast1mgQuote
EP426974STCE. coli1mgQuote
BP426974STCBaculovirus200ugQuote
MP426974STCMammalian Cell200ugQuote

Protein Information

SpeciesSalinispora arenicola (strain CNS-205)
UniProt IDA8LVI6
Gene NamevalS; Locus:Sare_0019
Protein NameValine--tRNA ligase
Region Expressed1-862
Expression Tag6xHis
Purity>90%
AA SequenceMPAKASLEGIEQVWSRVWEQNGTYRFDRSVTRDEVYSIDTPPPTVSGALHVGHVFSYTHA DAIARFQRMRGKMVFYPMGWDDNGLPTERRVQNYYGVQCDPSLPYDPGYTPPTEPPGRPQ PISRRNFVELCRNLTKIDEQAFEALWRHLGLSVDWSLTYATIDDRSRAISQRAFLRNLAR GEAYLADAPTLWDVSFRTAVAQAELEDRERQGHYYRLSFTSAAGDTIHVETTRPELLPAC VALVAHPDDKRYQHLFGSTAVTPVFRVPVPIKAHPLAQPDKGSGIAMICTFGDLTDVLWW RELALPTRPVMGRDGRLLPEPPPGITNAEAVAAYRTLAGLTAFSAKAKMVELLRASGDLA GDPQPTAQAVKFYEKGDKPLEIVTTRQWYVRNGGRNAQLRAALIRRGRELSWSPRFMRSR YENWVEGLAGDWIISRQRFFGVPIPVWYPLDDSGEPDYERPILPDDAALPVDPSSDTPTG YHDSQRHQPGGFMADPDVMDTWATSSLTPEIAGGWTVDDDLFGRVFPMDLRPQAHEIIRT WLFATMLRSHQEFDRLPWRTALLSGWILDPDRKKMSKSKGNSVVTPMSLLAEYGSDAVRY WAVSGRPGTDTAFDTGQMKIGRRLAIKILNATKFVLRFESPTLRAPHVAHVTEPLDRSML ARLADVVAAATTGFTQYDYTRSLECTEHFFWSFCDDYLELVKERAYGNPDDPAVRSAHAA LALALRTLLRLFAPMLPFVTEEAWSWWQEGSVHRASWPARQELVGDADGVDRANRIDETN GSDEAVEDLLGLASGVLAAIRRAKSEAKQSMRASVARLTLRGRASDLAAFALVSRDVRAA GVVRDVDTAEADVPLTPEITLG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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