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Recombinant Uroporphyrinogen decarboxylase (hemE)

Recombinant Uroporphyrinogen decarboxylase (hemE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP025678EZXYeast1mgQuote
EP025678EZXE. coli1mgQuote
BP025678EZXBaculovirus200ugQuote
MP025678EZXMammalian Cell200ugQuote

Protein Information

SpeciesPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
UniProt IDP95458
Gene NamehemE; Locus:PA5034
Protein NameUroporphyrinogen decarboxylase
Region Expressed1-355
Expression Tag6xHis
Purity>90%
AA SequenceMTALKNDRFLRALLKQPVDVTPVWMMRQAGRYLPEYRATRAKAGDFMSLCMNPELACEVT LQPLDRYPQLDAAILFSDILTIPDAMGQGLYFETGEGPRFRKVVSSLADIEALPVPDPEQ DLGYVMDAVRTIRRELNGRVPLIGFSGSPWTLATYMVEGGSSKDFRKSKAMLYDNPKAMH ALLDKLAQSVTSYLNGQIHAGAQAVQIFDSWGGSLSAAAYQEFSLAYMRKIVDGLIREHD GRRVPVILFTKGGGLWLESMAEVGAEALGLDWTCDIGSARARVGERVALQGNMDPSVLYA NPAAIRAEVARILAAYGKGTGHVFNLGHGITPEVDPAHAGAFFEAVHELSAQYHG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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