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Recombinant Uroporphyrinogen decarboxylase (hemE)

Recombinant Uroporphyrinogen decarboxylase (hemE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP025678STPYeast1mgQuote
EP025678STPE. coli1mgQuote
BP025678STPBaculovirus200ugQuote
MP025678STPMammalian Cell200ugQuote

Protein Information

SpeciesShewanella pealeana (strain ATCC 700345 / ANG-SQ1)
UniProt IDA8GZL8
Gene NamehemE; Locus:Spea_0427
Protein NameUroporphyrinogen decarboxylase
Region Expressed1-354
Expression Tag6xHis
Purity>90%
AA SequenceMAELKNDRYLRALLKQPVDRTPVWMMRQAGRYLPEYKATRAEAGDFMSLCKNQDLACEVT LQPLRRYDLDAAILFSDILTVPDAMGLGLYFEAGEGPRFERPTDTIDSIKKLCIPDPEDE LGYVMRAVSTIRRELKGEVPLIGFSGSPWTLATYMVEGGSSKAFEKIKKMAYEEPATLHM LLDKLADSVTLYLNAQVANGAQSLMIFDSWGGALSHHAYREFSLRYMQKIVDGLTRHADG RQVPVTLFTKGGGLWLESMAETGCDALGLDWTVDIGDARRRVGHKVALQGNMDPSVLYGT PERIHQEVDQILASYGEGTGHVFNLGHGIHQHVDPERAGSFINSVHELSPQYHK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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