Request QuoteCatalog Number: xP510733EOWSize: 0.2-1mg

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Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD)

Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP510733EOWYeast1mgQuote
EP510733EOWE. coli1mgQuote
BP510733EOWBaculovirus200ugQuote
MP510733EOWMammalian Cell200ugQuote

Protein Information

SpeciesEubacterium eligens (strain ATCC 27750 / VPI C15-48)
UniProt IDC4Z534
Gene NamemurD; Locus:EUBELI_00730
Protein NameUDP-N-acetylmuramoylalanine--D-glutamate ligase
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMDLKNKTVMVVGTGISGIGAVDLLNKVGADCILYDGNEKLDRQKVQEKLGDNKAEIIIGA FDEALLSKIDLLVISPGVPIDSPIVLTFKNAGIPVWGEIELAYNYDKGKIIAITGTNGKT TTTALVGQIIAAYNEKTFVVGNIGNSYTGEVLKTSEDSYTVAEISSFQLETVHEFHPIVS AILNITPDHLNRHHTMECYAWTKERISENQTKADTCVLNLEDKYLTDFAPECKANVVWFS SERKPSVGAYVDGEMIKYTDGTNDYDMLNVHDMNLLGKHNYENVCAAIAMTKAAGIPDDI IIEQVKKFKAVEHRIEYVATKNGVDYYNDSKGTNPEAAVKAIEAMVKPTILIGGGYDKGS EFDLYVKAFKDKVKLLVLIGQTSAKIADTCKKYGFENIEYADSMEQVVDICAKNAVSGDA VLLSPACASWGMFDNYEQRGRIFKDLVNNL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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