Request QuoteCatalog Number: xP462946BTASize: 0.2-1mg

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Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD)

Recombinant UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP462946BTAYeast1mgQuote
EP462946BTAE. coli1mgQuote
BP462946BTABaculovirus200ugQuote
MP462946BTAMammalian Cell200ugQuote

Protein Information

SpeciesBifidobacterium longum (strain DJO10A)
UniProt IDB3DQM9
Gene NamemurD; Locus:BLD_0179
Protein Name
Region Expressed1-481
Expression Tag6xHis
Purity>90%
AA SequenceMQVADKTVVIAGLGVSGTSLAEVLRERGTHVIGVDERKPEADLHSFDDVDWDHVDYVMSS PVFNPRTPFVLEAQRRGIPVMSEVEFAWQLRVNNERTGTPAPWIGITGTNGKTSTTEMTS EMLTACGLDAPTAGNIASGDMSMSLSRCATNPQHDVLCVELSSFQLHFTDSLALDCAAIT NIADDHLDWHGGRENYAADKSKVFHNAKRAIVYNAQDAKVSELAAEAQTAEGCRKVGFTL EAPQAGQIGIEDGWIVDRSGVAGGAVGESVRLAAITDFTHLAEPDGSLYPHLVADALTAL ALVLGLGADRDTALKALTSFKPGGHRIETVAEAAVEGGSVRFVDDSKATNGHAARASLSS FPAKSVIWIAGGLAKGSRFEDLVKDQAHTIKAAVIIGKDQQPMIEAFASQAPDIPVTIID PEDNDTVMDRAVEACGTYTAAGDIVLMAPACASMDQFKSYADRGNRFAAAAKTWSEVHGL H
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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