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Recombinant Tyrosine phenol-lyase (tpl)

Recombinant Tyrosine phenol-lyase (tpl) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518704DURYeast1mgQuote
EP518704DURE. coli1mgQuote
BP518704DURBaculovirus200ugQuote
MP518704DURMammalian Cell200ugQuote

Protein Information

SpeciesClostridium saccharolyticum (strain ATCC 35040 / DSM 2544 / NRCC 2533 / WM1)
UniProt IDD9R201
Gene Nametpl; Locus:Closa_0252
Protein NameTyrosine phenol-lyase
Region Expressed1-468
Expression Tag6xHis
Purity>90%
AA SequenceMDMMKFAPEPFKIKMVEPMGNLNKEERKDAIRTAGYNTFLLKSEECFIDLLTDSGTNAMS DRQWAGLMLGDEAYGGSRNFYHLEETVRELFGFKYVVPTHQGRGAENILSSLTIKPGDYV PGNMYFTTTRFHQEHNGATFRDVVIDEAHDPNAILDFKGNIDLNKFQALIDEVGAERIPY ICLAVTVNLAGGQPVSMANVKAVSELAHKHGIKVMFDATRCVENAYFIKTREKGYEDKSI KEIVHELFSYGDGCTMSGKKDCLTNIGGFLCMNDKDLYIRATGMVVQYEGMPTYGGMAGR DMEAMAIGLRESMEYNYISHRVNQIRYLGEKLDAAGVPMVKPSGGHAIFVDARAFLDHLD QKTDFPAQALAAAVYEFSGVRTMERGIISAGRDIKTGEDHVPKLETIRLTIPRRVYTYAH LDYVADAIIQLYQMRRDISGLKWVYEPAVLRFFTGRFEPKNGELIKGF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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