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Recombinant Type I iodothyronine deiodinase (Dio1)

Recombinant Type I iodothyronine deiodinase (Dio1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340619RAYeast1mgQuote
EP340619RAE. coli1mgQuote
BP340619RABaculovirus200ugQuote
MP340619RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDP24389
Gene NameDio1; aka: Itdi1, Txdi1
Protein NameType I iodothyronine deiodinase
Region Expressed1-257
Expression Tag6xHis
Purity>90%
AA SequenceMGLSQLWLWLKRLVIFLQVALEVATGKVLMTLFPERVKQNILAMGQKTGMTRNPRFAPDN WVPTFFSIQYFWFVLKVRWQRLEDRAEYGGLAPNCTVVRLSGQKCNVWDFIQGSRPLVLN FGSCTUPSFLLKFDQFKRLVDDFASTADFLIIYIEEAHATDGWAFKNNVDIRQHRSLQDR LRAAHLLLARSPQCPVVVDTMQNQSSQLYAALPERLYVIQEGRICYKGKPGPWNYNPEEV RAVLEKLCIPPGHMPQF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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