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Recombinant Type III iodothyronine deiodinase (Dio3)

Recombinant Type III iodothyronine deiodinase (Dio3) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP344617RAYeast1mgYP344617RA
EP344617RAE. coli1mgRPF989Ra01; EP344617RA
BP344617RABaculovirus200ugQuote
MP344617RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDP49897
Gene NameDio3; aka: Itdi3, Txdi3
Protein NameType III iodothyronine deiodinase
Region Expressed37-278
Expression Tag6xHis
Purity>90%
AA SequenceDFLCIRKHFLRRRHPDHPEPEVELNSEGEEMPPDDPPICVSDDNRLCTLASLKAVWHGQK LDFFKQAHEGGPAPNSEVVRPDGFQSQRILDYAQGTRPLVLNFGSCTUPPFMARMSAFQR LVTKYQRDVDFLIIYIEEAHPSDGWVTTDSPYVIPQHRSLEDRVSAARVLQQGAPGCALV LDTMANSSSSAYGAYFERLYVIQSGTIMYQGGRGPDGYQVSELRTWLERYDEQLHGTRPR RL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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