$75.00Catalog Number: PTN334-20ugSize: 20ug

TXN1 E.Coli

Thioredoxin E.Coli Recombinant

Recombinant Thioredoxin was purified from E. coli harboring its gene.

Accession

P0AA25

Amino acid sequence

HMSDKIIHL TDDSFDTDVLKADGAIL VDFW AEWCGPCKMIAPILDEI GKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGAL DANLA.

Biological Activity

TRX activity is assayed by measuring the change in absorbance at 650 nm at 25oC using 0.13uM bovine insulin containing 0.33mM DTT (pH 6.5).
The specific activity was found to be 3IU/mg.

Formulation

Each mg of protein contains 20mM phosphate buffer pH 7.4.

Physical Appearance

Sterile Lyophilized Powder.

Purity

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized TRX in sterile 18MΩ-cm H2O.

Source

Escherichia Coli.

Stability

TRX although stable at 4oC for 3 weeks, should be stored desiccated below -18oC.
Please prevent freeze thaw cycles.

Synonyms

Thioredoxin-1, Trx-1, trxA, fipA, tsnC, b3781, JW5856.

Quantity

$75.00

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