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Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA)

Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP509676ESQYeast1mgQuote
EP509676ESQE. coli1mgQuote
BP509676ESQBaculovirus200ugQuote
MP509676ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DFW7
Gene NamemnmA; Locus:PC1_1869
Protein NametRNA-specific 2-thiouridylase mnmA
Region Expressed1-368
Expression Tag6xHis
Purity>90%
AA SequenceMSDNSQKKVIVGMSGGVDSSVSAYLLQQQGYHVEGLFMKNWEEDDDTEYCSAATDLADAQ AVCDKLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDL GADYIATGHYVRRHDVDGKSRLLRGMDGNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVR KIAEELELATAKKKDSTGICFIGERKFTDFLARYLPAQPGPILSVDDNKPMGQHQGLMYH TLGQRKGLGIGGVKDGGEDPWYVVDKDVANNILYVAQGHEHPRLMSHGLIAQQLHWVDRQ PLTAELRCTVKTRYRQADIPCTVTPLGDDRITVRFDEPVAAVTPGQSAVFYLDDVCLGGG IIEERLQE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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