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Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA)

Recombinant tRNA-specific 2-thiouridylase mnmA (mnmA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505162DWZYeast1mgQuote
EP505162DWZE. coli1mgQuote
BP505162DWZBaculovirus200ugQuote
MP505162DWZMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
UniProt IDC4LJJ9
Gene NamemnmA; Locus:ckrop_1260
Protein NametRNA-specific 2-thiouridylase mnmA
Region Expressed1-372
Expression Tag6xHis
Purity>90%
AA SequenceMRVMAAMSGGVDSSVAAARLVAEGHEVIGVHLALASTPTTLRVGSRGCCSLEDSGDARRV ADALGIPFYVWDFSDRFREDVVDPFINSYEMGETPNPCLRCNEKIKFQALLDRGIALGFD AVATGHYARLKDGELRRAIDDNKDQSYVLGVLNDEQLAHCLFPLGDTRKPDIRKEAEAAG LVTASKPDSHDICFIPDGNTKAFLGNHIGVRSGDLKDQDGNVIGHHDGIYGFTIGQRKGL GLPGPAPDGKPRYVTDIDADTGTVTVGTRDDLRVGRLYADRLIRLDEHEGSTQECQVQVR AHGGVVDATVTIDDGVATIDLHSPLTGVARGQAAVIYRPDPDGDIVLGSGTICDTVSVSK LQPTTAGASSNS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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