Request QuoteCatalog Number: xP520597TNHSize: 0.2-1mg

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Recombinant tRNA pseudouridine synthase Pus10 (pus10)

Recombinant tRNA pseudouridine synthase Pus10 (pus10) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520597TNHYeast1mgQuote
EP520597TNHE. coli1mgQuote
BP520597TNHBaculovirus200ugQuote
MP520597TNHMammalian Cell200ugQuote

Protein Information

SpeciesThermosphaera aggregans (strain DSM 11486 / M11TL)
UniProt IDD5TZL3
Gene Namepus10; Locus:Tagg_0031
Protein NametRNA pseudouridine synthase Pus10
Region Expressed1-441
Expression Tag6xHis
Purity>90%
AA SequenceMGGSSISTPLDFNKIVEDVEKTLSKYPLCDHCLGRFFAKYGLGLSNRERGSSLKTMLGFK LYDDYSSKRIGRDSLLALAENAGEPLTRMVEKLFAENVNPKTCFICGGRISDEWLDSVAE AVYEKLKEASVTRFVVGVKLGRNLREKELQLVTETGFTRAESLKNEIKREVGKKVMAKYG LVPDFEKPEATAIVKLDENFNYRVELVITPVLLKGVYNKRGRNISHVPWLAKQGGRKYPL SIQEYLESRLADPFKAKKVKIHAAGREDVDARTLGPGRPLVIEIVEPLVRSYDIEDVNKL IRSDLVEVRVHKPASRRDIELLKQTSREKRKVYRLLVVSSTPISEAQLNELEAYFNDIAI QQRTPIRILTRKKDVLRVRKVYEVRTKPVSSTSFEALVYCDGGLYVKELVHCDQGRTNPC FASILNTELKPVELDVLYIEH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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