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Recombinant tRNA pseudouridine synthase Pus10 (pus10)

Recombinant tRNA pseudouridine synthase Pus10 (pus10) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516717MSQYeast1mgQuote
EP516717MSQE. coli1mgQuote
BP516717MSQBaculovirus200ugQuote
MP516717MSQMammalian Cell200ugQuote

Protein Information

SpeciesMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
UniProt IDD9PYH9
Gene Namepus10; Locus:MTBMA_c17080
Protein NametRNA pseudouridine synthase Pus10
Region Expressed1-402
Expression Tag6xHis
Purity>90%
AA SequenceMDVQERLDAILDITDSKICPHCLGRRFSDVMEGPGNRLRGERLVEKFSLHLEGPCLVCGD VFERLDEAALRVREKVDDLNLEYSSVLVGTRLPDDVLRIDEEIDRRLGIQVEGIKREVNR ELGKRVTSILRCPADFESPDLVITVDLRGQIRVHVQINPIFIEGRYRKLVRGIPQTKWPC RSCRGRGCSRCDYTGKMYPTSVEELISEPVLEATGGSDSKFHGSGREDVDVRMLGTGRPF VLEIKEPAIRTPDLRALEDEINRRARGMVEVADLRFSSRNRKVELKESSRKKYKVYRAIV ELEGAVSDEDLVKLEKLDLIRQRTPLRVSHRRADRIRERRVLEISWKRLDDHLELIIKAE GGLYIKELISGDSGRTEPSVSSILGVPARCASLDVLEVGEPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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