Request QuoteCatalog Number: xP513290ENUSize: 0.2-1mg

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Recombinant tRNA (mo5U34) -methyltransferase

Recombinant tRNA (mo5U34) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513290ENUYeast1mgQuote
EP513290ENUE. coli1mgQuote
BP513290ENUBaculovirus200ugQuote
MP513290ENUMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain K12 / MC4100 / BW2952)
UniProt IDC4ZQF5
Gene NamecmoB; Locus:BWG_1685
Protein NametRNA (mo5U34) -methyltransferase
Region Expressed1-323
Expression Tag6xHis
Purity>90%
AA SequenceMIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSNAVEFLPEIKPYRLDLL HSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNIDTEWRSDWKWDRVLPHLSD LTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQFEAVRKLLGNDQRAHLLPLG IEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVNEGELVLETLVIDGDENTVLVPG DRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVSVTTTEEQRRTEWMVTESLADFLDP HDPGKTVEGYPAPKRAVLIARKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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