Request QuoteCatalog Number: xP504081RMXSize: 0.2-1mg

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Recombinant tRNA (Ile) -lysidine synthase

Recombinant tRNA (Ile) -lysidine synthase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504081RMXYeast1mgQuote
EP504081RMXE. coli1mgQuote
BP504081RMXBaculovirus200ugQuote
MP504081RMXMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia peacockii (strain Rustic)
UniProt IDC4K162
Gene NametilS; Locus:RPR_02390
Protein NametRNA (Ile) -lysidine synthase
Region Expressed1-533
Expression Tag6xHis
Purity>90%
AA SequenceMLYEKFEYNINNLIGNFGLSKMSIAVSGGSDSVALLYLANIWAEKNNIELFVISVDHNLR EQSKQETHYIQNISNSLNRKHYSLSFDHQNNFSNLQERAREGRYDLMTNLCLELDILVLL TAHHEDDYVENFCLRLERNSGIFGLSSSNINWYNNIHIIRPLYNIPKSELVEYLVSHNIK WFEDESNSSDKYRRNVIRQKLAKGADYIRHFSKPVYREEFKGDTERSTAAYTSVGEEALL RGPVKLTVSSRGLTTVKTIKSTNNFSIFNWIPWSSHGMTEVKLIHATMPREDASTGTASK LSLEAKCGKMSKAAIISQQLKTNKRIENEFKPELISAIAEAVKIFEYGFAFLDLVKFDKF SNEVKVQIINFLLIIISGQSRAARFYSVEPILKLITQDVNFKNTLHGCIIKRIQNELLIY REFGKKLPESKILLDKSVIWDNRFCITKNQETPNCFVTHLSLKDYKIIKKQLDLEPLKNL SCKNHNAVLLTLPIIKILEKVIAIPHISYYDNDMWNFEVSFSPNFVSRFTHFC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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