Request QuoteCatalog Number: xP513897EKFSize: 0.2-1mg

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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513897EKFYeast1mgQuote
EP513897EKFE. coli1mgQuote
BP513897EKFBaculovirus200ugQuote
MP513897EKFMammalian Cell200ugQuote

Protein Information

SpeciesEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
UniProt IDC8VJ35
Gene Nametrm5; ORFs:AN10361
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed1-478
Expression Tag6xHis
Purity>90%
AA SequenceMAQSTEDNTRRPRPSDLPEMFRPPVNRAMRVLDRSFFKKTVPLSAATIFKNSDISRVRRA LEQSKDILALPRLNTIQDIKQDDVVKKCLLLKETIRHDDTATWSPTINELVDNGVVGVGP FDLTLDYDYWLHSDIISAVLPEELLEEVPQGFTQVGHVAQLNLREQFIPWRHLIAQVLLD KNPTLRTVIRKTEDVGSQSEFRTFPYELLAGDSDMNVIQHEQDCEFRFDFSRVYWNSRLH TEHQRLVDLFKPGEMVCDVMAGVGPFAIPAGKKKIFVWANDLNPHGYEVMQDAVKRNKVF KFVTPFNQDGRSFIRWSARALQKYDPVTVTIQPRTKRTRDASGQVKETQPPLEVYTRPKV FHHYVMNLPGNALEFLDAFIGVYAGCEELFEPHTKEQLPMVHVYCFSGHSENEVDDHIDI CKRMSERLEYPITVEDRVGGAGNTELELSIHNVRLVSPNKQMFCASFRLPRAVAFRKK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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