Request QuoteCatalog Number: xP522279NHASize: 0.2-1mg

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Recombinant tRNA (guanine (37) -N1) -methyltransferase

Recombinant tRNA (guanine (37) -N1) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP522279NHAYeast1mgQuote
EP522279NHAE. coli1mgQuote
BP522279NHABaculovirus200ugQuote
MP522279NHAMammalian Cell200ugQuote

Protein Information

SpeciesNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
UniProt IDF5HAU9
Gene Nametrm-5; ORFs:NCU09311
Protein NametRNA (guanine (37) -N1) -methyltransferase
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMSQSEKENQGSGEGKTQDMAIFSPPVVRSGAGALNRALFTKTVNLAAAAVNDNRLISKYR KELEHSKELLRQDRLSPIVNHPDKALADQGKKCLLLSPNVKAPEPETWGAALKEGVQKKE LSVIPYELQLNYDYWTYHDIITSILPEELHDDIPSGFNTAGHVAHMNLRERYIPYKKVIA EVILDKTTNIRTVINKVDNVGAESEFRTFQYEVLAGPDDMQVQVTENACSFEFDYSKVYW NSKLEAEHRRLINMFEPGEVVCDVMAGIGPFAVPAGKKGVFVWANDMNPESNKYMQVAIN RNKVSQYVRPICEDGRTFIHHAADSVLEAHKNGEHVLIAPKPPSRAKKAPKPEPKRVDIP PTISHFVMNLPATAIEFLGCYRGVYAGHEDLFSAESGRKLPLVHVHCFSFKADDETPLND ICERITKYLGFPVKPGNPDVEGEVAVHDVRDVAPAKRMFCASFRIPREVAFAERV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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