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Recombinant Trigger factor (tig)

Recombinant Trigger factor (tig) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP505172DWZYeast1mgQuote
EP505172DWZE. coli1mgQuote
BP505172DWZBaculovirus200ugQuote
MP505172DWZMammalian Cell200ugQuote

Protein Information

SpeciesCorynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717)
UniProt IDC4LJV9
Gene Nametig; Locus:ckrop_1373
Protein NameTrigger factor
Region Expressed1-495
Expression Tag6xHis
Purity>90%
AA SequenceMKSSVEQQSATRVKLTVEVPFDELQPEIDQAYRSLAQQVTLPGFRKGKVPPRVLEARLGR GAILDQAINNMLPSRYSQAVEEHDLKVLTQPTIDITRLEDNELVEFTAEADVRPEIDLPD FSTIEVEVDPLVADDAAVDAELDNLRARFGTLKAVERPVQKDDFVSIDLSATVGGEPVDE ATTEGLSHQVGNGELIEGLDDALEGMKVDESKTFKSNLVAGDHEGEEAEVTVTVKSLKER ELPEADDDFAQLASEFDTIGELRESLAKQVEENKKAEQASSIRDKVLEKALEKTDFELPE AVVEEQVKGQLNQLLQQFNGDEKAFDQLLAAQGSSREQFDRDTRSSAEEAVRTQLFLDTL ADELQPEVSQQEFTEHIMFTAQRYGMEPQQFMQAIQASDQIGALYADVRRGKALAESICT VSVKDTDGNEIDPKEYFGEEENTDETSTGSADAEASENSEATPSGTETEESAEFDQAAEA PVATPTDDDSENAEK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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