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Recombinant Trigger factor (tig)

Recombinant Trigger factor (tig) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504289HUGYeast1mgQuote
EP504289HUGE. coli1mgQuote
BP504289HUGBaculovirus200ugQuote
MP504289HUGMammalian Cell200ugQuote

Protein Information

SpeciesHamiltonella defensa subsp. Acyrthosiphon pisum (strain 5AT)
UniProt IDC4K4P3
Gene Nametig; Locus:HDEF_0810
Protein NameTrigger factor
Region Expressed1-440
Expression Tag6xHis
Purity>90%
AA SequenceMQVLVETTEDLKKRATITLFSDDIEKAIKDKVVKTAKTAKMDGFRKGKVPINLIEQRYGE SIQQDILIDFMQDHFSKTILQEKLNLAGRPQYLPGKYIKNENYTYSVEFEVYPEIKLKDL ELIEVEKPIVSLNDDDIDKMLEKLRHQKKTWQEIPDLPAEMGDRVTFDFTGSVDGEKFEG GTASDFVMNLGEGNMIPGFEEGIVGHKMSEEFNITLTFPDEYHSESLKGKESQFLIHLKK IEKFQLPEINEEFIKNFDLPEESSIEALRAEIRKNMQRELTKAIRKRIKEQTITSLCDLN KTKVPLSLIEEEIDALRTKAASYSDKKDIIKELPRSVFAEKAESRVLVGLLLTEVIDRNK LTVDENKVQVFLEEIAEAYENPKETIEFYKNNKKLMNNIYNLALEEEAIQVLLKNAKITE KPISFSEFMKQIHETDTAND
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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