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Recombinant Trigger factor (tig)

Recombinant Trigger factor (tig) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP504143RMXYeast1mgQuote
EP504143RMXE. coli1mgQuote
BP504143RMXBaculovirus200ugQuote
MP504143RMXMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia peacockii (strain Rustic)
UniProt IDC4K233
Gene Nametig; Locus:RPR_04970
Protein NameTrigger factor
Region Expressed1-445
Expression Tag6xHis
Purity>90%
AA SequenceMGITVLKNEGLDFHARISTPLSEIDDDIQKELLDLTKKVKIAGFRAGKVPVSIVKKKYGT SVRNDIIERRINHSVNHVIKEHNLNIIGRPKIEELQNESDKALEFTVKIELLPKITIPDL KKISLDRPKLEVNSKDVEEQLEKLAALTKNYTKESKAKIKDGDQVTIDAIGYIKEKAFED GKLNDFKVIIGSNALIPGFEKQLIGSKTGSEVDVNVTFPENYHAKDLAGKDARFVVQIKA VHTAEPTVIDDEFAKKFQSNSLEELRTHFTKQIENESEEAINTIMKMNLFDKLEKLLDFD VPESLLEQEKNILKSGTDKNEQDESLLKDKSSKEITAYYNKLALRRVRIGLLLAEYAKSK NLQLEPDDLRKVIMQQARNFPGQENMIFDFYKNNPRAIEGLKGPALEDKAVQYIFNHEIK LKEKKYTKEELEKYLEAEEQRITLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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