One mg of Recombinant Human Transferrin will bind to approximately 2 micrograms of Fe.
Animal component-free.
Well defined and consistent.
Universal iron carrier in cell culture.
Replaces plasma-derived human and bovine transferrin.
Replaces iron chelators.
Recombinant Human Transferrin has increased the growth of numerous cell types including hybridoma and primary cell lines. The optimal concentration of Recombinant Human Transferrin for cell culture differ with the cell line. The reported effective concentrations for hybridoma and Vero cell lines range from 5mg/liter to 20mg/liter. Effective concentrations for primary cells range between 5mg/liter to 100mg/liter.
The protein (1mg/ml) was lyophilized with no additives.
Sterile Filtered lyophilized (freeze-dried) powder.
Purity as determined by SDS-PAGE is 95%.
Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 5mg/ml to 20 mg/ml in PBS, though others can be used as well. Please try to avoid the formation of bubbles when dissolving the protein. Sterile filter through 0.2um filter.
Lyophilized Transferrin although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Transferrin Human Recombinant should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Serotransferrin, Transferrin, Siderophilin, Beta-1-metal-binding globulin, TF, PRO1557, PRO2086, DKFZp781D0156, Holo Transferrin, HTF.
