$148.20Catalog Number: PYT422-50ugSize: 50ug

TNFR Human

Tumor Necrosis Factor Receptor Human Recombinant

Tumor Necrosis Factor Receptor Human Recombinant produced in CHO is a dimeric, glycosylated, polypeptide chain consisting of the extracellular ligand-binding portion of the human 75 kilodalton (p75) tumor necrosis factor receptor (TNFR) linked to the Fc portion of human IgG1. The Fc component of TNFR contains the CH2 domain, the CH3 domain and hinge region, but not the CH1 domain of IgG1. It consists of 934 amino acids and has an apparent molecular weight of approximately 150 kilodaltons.
The TNFR is purified by standard chromatographic techniques.

Accession

P20333

Biological Activity

Potency is determined by its ability to neutralize TNF-alpha mediated growth inhibition of A375 cells, corresponding to a Specific Activity of 17,000,000 IU/mg.

Formulation

Each mg contains 1.6mg mannitol, 0.4 mg sucrose and 48ug tromethamine.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by
(a) Analysis by RP-HPLC.
(c) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized TNFR in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Chinese Hamster Ovarian Cells (CHO).

Stability

Lyophilized Tumor Necrosis Factor Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution TNFR should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Tumor necrosis factor receptor superfamily member 1B,Tumor necrosis factor receptor 2, TNF-R2, Tumor necrosis factor receptor type II, p75, p80 TNF-alpha receptor, CD120b antigen, Etanercept, TBPII, TNFBR, TNFR80, TNF-R75, p75TNFR, TNF-R-II.

Quantity

$148.20

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