$176.70Catalog Number: MAB124-500ugSize: 500ug

TNF a Antibody

Tumor Necrosis Factor-alpha, Mouse-Anti Human
Monoclonal Antibody

Monoclonal antibodies provide the best target specificity and affinity. It is also advantagous for long term projects as there is little lot to lot variation, where polycolonal antibodies will vary significantly with different preparations due to immunogen preparation and animal responses.

Applications

Direct ELISA, Western Blot, Immuneprecipitation, Intracellular staining.

Clone

NYRhTNFa-E2.

Ig Subclass

Mouse IgG1.

Immunogen

r.Human TNF-a.

Note

This antibody will bind very well to protein A in a buffer (PBS) containing high salt concentration (3M Nacl).

Protein concentration

1mg/ml in PBS (after reconstitution).

Purification Method

ion exchange.

Reconstitution

Reconstitute with sterile H2O. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.

Shipping Conditions

Antibody is shipped lyophilized at ambient temperature.

Storage

In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Synonyms

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2.

Titer

In direct ELISA, using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories) 1:10,000 dilution will yield 0.7 O.D within 10 minutes.

Type

Mouse Anti Human Monoclonal.

Quantity

$176.70

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