$90.00Catalog Number: PYT716-2ugSize: 1ug

TGF b 1 Human Recombinant

Transforming Growth Factor-Beta 1 Human Recombinant

TGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa. The TGFB1 is purified by proprietary chromatographic techniques.

Accession

P01137

Amino acid sequence

ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.

Biological Activity

The ED50, as measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells, was found to be 0.126 ng/ml, corresponding to a specific activity of 7,936,508 Units/mg.

Formulation

Lyophilized from a concentrated (1mg/ml) solution containing 10mM HCl & 50ug BSA.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized TGFB1 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Source

Human 293 cells.

Stability

Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution TGFB1 Human should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Synonyms

Transforming growth factor beta-1, TGF-beta-1, CED, DPD1, TGFB, TGF-b 1, LAP, TGFB1.

Quantity

$90.00

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