Transforming Growth Factor-Beta 3 Human Recombinant
TGF-b3 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing 112 amino acids and having a molecular mass of 25,445 Dalton.
The TGF-b 3 is purified by standard chromatographic techniques.
Accession
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Asp-Thr-Asn.
Biological Activity
The ED50 as determined by the cell toxicity assay using the WHO Standard 98/608 as a direct comparison is < 0.05ng/ml corresponding to a specific activity of 20MU/mg.
Formulation
The protein solution contains 20% Ethanol and 0.12% Acetic acid.
Physical Appearance
Sterile Filtered clear solution.
Protein content
Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard.
Purity
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Source
Stability
TGF-beta 3 although stable at room temperature for 3 weeks, should be stored at 4oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Synonyms
Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3.
