$90.00Catalog Number: PYT005-5ugSize: 5ug

TFF3 Human

Trefoil Factor-3 Human Recombinant

TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa.
TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.

Accession

Q07654

Amino acid sequence

EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK
PLQEAECTF

Biological Activity

Determined by its ability to chemoattract human MCF-7 cells using a concentration 1.0-10.0 ng/ml.

Formulation

Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized TFF3 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution TFF3 should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor.

Quantity

$90.00

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