TEV

$285.00Catalog Number: PTN585-1,000IUSize: 1,000IU

Tobacco Etch Virus Protease Recombinant

Recombinant TEV Protease (rTEV) is a site-specific protease purified from E. coli. The protease can be used for the removal of affinity tags from fusion proteins. The seven-amino-acid recognition site for rTEV is Glu-Asn-Leu-Tyr-Phe-Gln-Gly with cleavage occurring between Gln and Gly. The optimal temperature for cleavage is 30^oC; however, the enzyme can be used at temperatures as low as 4^oC.
The rTEV contains His tag.
The rTEV is purified by proprietary chromatographic techniques.

Accession

Q0GDU8

Activity

10,000 Units/1mg.

Cleavage Conditions

A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat-labile, then 4^oC incubations are recommended. Reactions at 4^oC will require longer incubation times and/or more rTEV.

Formulation

The rTEV contains 0.50 M Tris-HCl pH 8.0, 10mM DTT and 5 mM EDTA.

Physical Appearance

Sterile liquid formulation.

Purity

Greater than 90.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Source

Escherichia Coli.

Stability

Store rTEV at -70^oC for long term or at -20^oC for< 6 months. Please prevent freeze-thaw cycles.

Synonyms

rTEV, TEV, P1 protease.

Unit Definition

One unit of rTEV cleaves >85% of 3 ug control substrate in 1 h at 30^oC.

Quantity