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Recombinant 2-succinylbenzoate--CoA ligase (menE)

Recombinant 2-succinylbenzoate--CoA ligase (menE) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP332279HTAYeast1mgQuote
EP332279HTAE. coli1mgQuote
BP332279HTABaculovirus200ugQuote
MP332279HTAMammalian Cell200ugQuote

Protein Information

SpeciesHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
UniProt IDP44565
Gene NamemenE; Locus:HI_0194
Protein Name2-succinylbenzoate--CoA ligase
Region Expressed1-452
Expression Tag6xHis
Purity>90%
AA SequenceMYPWQDFAIQPDFSDKIALRTTQGDMLTWIELTTKINQTVAFLQKKGVNAESAVAFVGKN SEKILFLYLATIQLGAKVLGINPAFPQEKIAKLCEFYQIDFCFYDKDLLNLQEIDVFTQK ADFFRPATMTLTSGSTGLPKAVVHNVQAHLDNAKGVCNLMKFDCNQSWLLSLPLYHVSGQ GIVWRWLYCGAQLHFPEDDFYASLLKTTHVSLVPTQLQRLLDYLQENPSISFATRHILLG GAHIPTELTQNMLKYGIETYSGYGMTEMASTVFAKKSDRKQGVGQPLLGREYCLVNDEIW LKGAGLAMGYWKDRQIVPLTNNQGWIQTKDKGIWQEGELVIIGRLDNMFISGGENIQPEE IEQVIIQHSSVNQVFVLPQKNKEFGQRPVALVDFNEPFSKSAVENLMFFLQDKLARFKQP IAYYPLPLMLEKGIKISRKQLADWLAKRDEIN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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