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Recombinant 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH)

Recombinant 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (menH) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP748946PIJYeast1mgQuote
EP748946PIJE. coli1mgQuote
BP748946PIJBaculovirus200ugQuote
MP748946PIJMammalian Cell200ugQuote

Protein Information

SpeciesPhotorhabdus luminescens subsp. laumondii (strain TT01)
UniProt IDQ7N2K4
Gene NamemenH; Locus:plu3072
Protein Name2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase
Region Expressed1-278
Expression Tag6xHis
Purity>90%
AA SequenceMSLVCRKFNGYNDRPWLVWLHGLLGCGDEWLPLVNLCAQHPSLVIDLPGHGDSTDVRVKD LLEMSCLLSETLAEQRISQYWLIGYSLGGRIAMHHACYGDIRGLCGLLIEGGNPGLFSQQ ERNNRLEHDRNWAGRFRSQPIEQVLSDWYQQPVFSDLLPEQRQQLIKVRRHNNGDGVANM LENTSLGHQPWLVPVLQQLTLPFVYLCGENDKKFQHLAKRCALPLQTIPRVGHNAHRANA VAFAAVVNHFLSLFNKEYEYDLPERRRTLFPNCMAGLL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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