Request QuoteCatalog Number: xP492512DJJSize: 0.2-1mg

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Recombinant 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD)

Recombinant 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (menD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP492512DJJYeast1mgQuote
EP492512DJJE. coli1mgQuote
BP492512DJJBaculovirus200ugQuote
MP492512DJJMammalian Cell200ugQuote

Protein Information

SpeciesDesulfitobacterium hafniense (strain DCB-2 / DSM 10664)
UniProt IDB8FTY8
Gene NamemenD; Locus:Dhaf_0470
Protein Name2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase
Region Expressed1-550
Expression Tag6xHis
Purity>90%
AA SequenceMTNYIAALVDELYQLGVREAVISPGSRSTPLSVLFCEYGFQVYVGIDERSAGFFALGIAK EKERPVVLVCSSGSAVAHYFPAIVEAKHSHVPLIVLTADRPPELRQVGAPQTIDQIKFYH DYAKYFEELALPEEREGMYRYVRGVMRKAYVSSLDQGYGVAHINIPLREPLSPDLDGVDF TAGRLDYPFAWKTGEKGLTMDSSVFQDKKGIIICGGDPYADYHREVLELAERLKAPLLAD PLANFRNDDHPLIMDCYDAFLKSDAVKVELKPEFIIHFGQTPVSKRLQNFTAMHQDVLYF QVNDYFQYRDPSLSISRYLVASPKAFARSVKVHNTDDGYSGRWQHYQARMRRRLNLAQDE EELFEGKLVQKLQDSLPEGSRLFVANSMAIRDVDYFLEARRQRIKVLGNRGANGIDGTVS TALGVATSGHPTVLLTGDLAFFHDLNGLLIGKNHGLNLIIVLLNNNGGAIFKYLPQSENK YFELLFMTPHGMDFSGLKTLYDLTYYEPVDYESFAQNFQEALTLSGVKVLNVKIDARLSK ELHDRLTNLD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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