Western blot with PBC sera or monoclonal anti-hexa-His-tag antibody.
0.5-0.9 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for biotinylation and iodination.
Sp100 is supplied in 16mM HEPES buffer pH-8.0, 400mM NaCl, and 20% glycerol.
Binds IgG-type human auto-antibodies.
Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot test.
Greater than 80% as determined by SDS-PAGE.
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time. Avoid multiple freeze-thaw cycles.
Nuclear dot-associated Sp100 protein, SP100 nuclear antigen, Speckled 100 kDa, SP100-HMG nuclear autoantigen, FLJ00340, Lysp100b.
