$75.00Catalog Number: PTN648-5ugSize: 5ug

SOD1 Human

Superoxide Dismutase 1 Human Recombinant

Recombinant Human Cu/Zn Superoxide Dismutase produced in E.Coli is a single monomeric non-glycosylated polypeptide chain containing 154 amino acids and having a total molecular mass of 15.9 kDa.
The SOD1 is purified by proprietary chromatographic techniques.

Accession

P00441

Amino acid sequence

MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVIGIAQ.

Biological Activity

Specific activity is > 90 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume.

Formulation

SOD1 0.2um filtered solution contains 20mM Tris-HCl pH-7.5 and 10% glycerol.

Physical Appearance

Sterile Filtered colorless solution.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Source

Escherichia Coli.

Stability

SOD1 although stable 4oC for 4 weeks, should be stored desiccated below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Superoxide dismutase [Cu-Zn], EC 1.15.1.1, SOD1, SOD, ALS, ALS1, IPOA.

Quantity

$75.00

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