$148.20Catalog Number: PYT597-25ugSize: 25ug

SHH Mouse

Sonic Hedgehog Mouse Recombinant

Sonic Hedgehog Recombinant Mouse produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids and having a molecular mass of 19.8 kDa. The Mouse Sonic Hedgehog is 99% homologous to the human gene.
Cysteine at position 25 has been substituted with Ile.
The Sonic HedgeHog is purified by proprietary chromatographic techniques.

Accession

Q62226

Amino acid sequence

MIIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG.

Biological Activity

Determined by it¡¯s ability to induce alkaline phosphatase production by C3H10T1/2 fibroblasts, with an expected ED50 in the range of 50-250ng/ml corresponding to a specific activity of 4,000-40,000IU/mg.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM sodium Phosphate buffer pH-7.5.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Sonic HedgeHog in sterile 18MΩ-cm H2O at a concentration of 0.1mg-0.5mg per 1ml.

Source

Escherichia Coli.

Stability

Lyophilized Mouse Sonic HedgeHog although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Sonic HedgeHog should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.

Synonyms

SHH, HHG-1, HHG1, Sonic hedgehog protein.

Quantity

$148.20

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