The antibody was developed using a synthetic peptide from the putative SARS Spike glycoprotein(Accession no. NP_828851) corresponding to amino acids 19-35.
This monoclonal antibody can be used for detection of SARS Spike protein in western blot analysis at 1-3 ug/ml. The antibody was tested on a cell line transfected with full-length cDNA coding for the SARS Spike protein with a predicted molecular weight of 139 kDa.
It has recently been shown that SARS (severe acute respiratory syndrome) is caused by a human coronavirus. Human coronaviruses are the major cause of upper respiratory tract illness, such as the common cold, in humans. Coronaviruses are positive-stranded RNA viruses, featuring the largest viral RNA genomes known to date (27-31 kb). The first step in coronavirus infection is binding of the viral spike protein, a 139-kDa protein, to certain receptors on host cells. The spike protein is the main surface antigen of the coronavirus. The glycosilated spike protein (as well as the nucleocapsid protein) can be detected in infected cell culture supernatants with antisera from SARS patients.
100 ug in 200 ul PBS containing 0.2% gelatin and 0.05% sodium azide.
Store at 4C, stable for 6 months. For long-term storage, store at -200C.
Western blot analysis of SARS Spike in
(a) transfected mouse melanoma cell lysate and (B) untransfected cell lysate.
Mouse antibody Monoclonal.
