Request QuoteCatalog Number: xP340833SXBSize: 0.2-1mg

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Recombinant RNA polymerase sigma-54 factor (rpoN)

Recombinant RNA polymerase sigma-54 factor (rpoN) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340833SXBYeast1mgQuote
EP340833SXBE. coli1mgQuote
BP340833SXBBaculovirus200ugQuote
MP340833SXBMammalian Cell200ugQuote

Protein Information

SpeciesSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
UniProt IDP26979
Gene NamerpoN; aka: glnF, ntrA; Locus:STM3320
Protein NameRNA polymerase sigma-54 factor
Region Expressed1-477
Expression Tag6xHis
Purity>90%
AA SequenceMKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALENNPLLEQTDLHDEIDTQQ PQDNDPLDTADALEQKEMPEELPLDASWDEIYTAGTPSGPSGDYIDDELPVYQGETTQSL QDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVSLDEIRESMGDVEVDLDEVEAVLK RIQRFDPVGVAAKDLRDCLLIQLSQFDKSTPWLEEARLIICDHLDLLANHDFRTLMRVTR LKEEVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGRWTVELNSDSIPRLQIN QHYAAMCNSARNDADSQFIRSNLQDAKWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEE YMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELKYFFSSHVNTEGGGEASSTAI RALVKKLIAAENPAKPLSDSKLTSLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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