Request QuoteCatalog Number: xP516623TOFSize: 0.2-1mg

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Recombinant RNA-directed RNA polymerase catalytic subunit (P2)

Recombinant RNA-directed RNA polymerase catalytic subunit (P2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516623TOFYeast1mgQuote
EP516623TOFE. coli1mgQuote
BP516623TOFBaculovirus200ugQuote
MP516623TOFMammalian Cell200ugQuote

Protein Information

SpeciesThogoto virus (isolate SiAr 126) (Tho)
UniProt IDO41353
Gene NameP2
Protein NameRNA-directed RNA polymerase catalytic subunit
Region Expressed1-710
Expression Tag6xHis
Purity>90%
AA SequenceMNLFTPRSEINPTTTQELLYAYTGPAPVAYGTRTRAVLENIIRPYQYFYKEPNVQRALDI KTGCKEPEDINVEGPSSGFHTASVLKLADNFFRKYRPAMEKLKYWILVKLPKLKYAELSK GRQTYSFIHKRNLPAPIALEETVEFLEQNLRRKIGPTLLSYCQAIADVMELDETTYEGAR DPRPWDIQLEEIDSDEEDPLFRQVGREETYTIKFSREELWDQMRTLNTMCKHLERGRLNR RTIATPSMLIRGFVKIVEDAAKEILENVPTSGVPVGGEEKLAKLASKQTFHTAVTGELSG DQEKFNECLDPDAMRLMWTVFLRKLGCPDWIMELFNIPFMVFKSKLADMGEGLVYTKGKL TDRKPLGEMPSEFDDLVRNVVGNSISCRLGMFMGMYNLTSTLLALISIEREELTGSHVES SDDFIHFFNCKTHEEMFKQAETLRLTLKLVGINMSPSKCILISPAGIGEFNSKFHHRDFV GNVATELPALVPNGTNPMTDLAMGLNVIKHSVNTGQMNLCTGALAMRIFNHAYKYAYMAL GVTRRTRFMEENAITPLLTNQGASPVHSFSTMHLDEVALRRHLGLLDEETLRRILNPNNP VTQKGDPSMFFRIENKMPQIMEDYSVPSCFKYTLSRNRTIQDKPHKALLNKEERYQRVTS IINKLFPEVLIQEASAPGTVRESLKRRLELVVERSDLDEERKKRILSRIF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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