Request QuoteCatalog Number: xP520339DZHSize: 0.2-1mg

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Recombinant RNA-directed RNA polymerase 2a (ORF2a)

Recombinant RNA-directed RNA polymerase 2a (ORF2a) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520339DZHYeast1mgQuote
EP520339DZHE. coli1mgQuote
BP520339DZHBaculovirus200ugQuote
MP520339DZHMammalian Cell200ugQuote

Protein Information

SpeciesCucumber mosaic virus (strain NT9) (CMV)
UniProt IDO40977
Gene NameORFs:ORF2a
Protein NameRNA-directed RNA polymerase 2a
Region Expressed1-857
Expression Tag6xHis
Purity>90%
AA SequenceMAFSAPTFSLANLLNGSYGVDTPEEVERVRSEQREEAAAACRNYRPLPAVDVSESVTEDA HSLRTPDGAPSEEVSVEFVTYGAEDYLEKSDDELSVAFETMVKPMRIGQLWCPAFNKCSF ISSIAMARALLLAPRTSHRTMKGFEDLVAAIYTKSDFYYDEECEADDIQIDVSSRDVPGY SFEPWSRTSGFEPPPICEACDMIMYQCPCFDFNALKKSCAERTFADDYVIEGLDGVVDNA TLLSNLGPFLVPVKCQYEKCPTPTVAIPPSLNRATDRVDINLVQSICDSTLPTHSNYDDS FHQVFVESADYSIDLDHVRLRQSDLIAKIPDSGHMIPVLSTGSGHKRVGTTKEVLTAIKK RNADVPELGDSVNLSRLSKAVAERFFISYINGNSLASSNFVNVVSNFHDYMEKWKSSGLS YDDLPDLHAENLQFYDHMIKSDVKPVVSDTLNIDRPVPATITYHKKGITSQFSPLFTALF ERFQRCLRERIILPVGKISSLEMAGFDVKNKHCLEIDLSKFDKSQGEFHLMIQEHILNGL GCPAPITKWWCDFHRFSYIRDRRAGVGMPISFQRRTGDAFTYFGNTIVTMAEFAWCYDTD QFEKLLFSGDDSLGFSVLPPVGDPSKFTTLFNMEAKVMEPAVPYICSKFLLSDEFGNTFS VPDPLREVQRLGTKKIPYSDNDEFLFAHFMSFVDRLKFLDRMTQSCIDQLSLFFELKYRK SGAEAALMLGAFKKYTANFQSYKELYYSDRRQCELINSFSCVELRIERSSSTKQRKKKDG IERRRSDKRRTPTGSYGGGEKTETKVSHEESTGTRSQKSQREGAFKSQIVPLPTILSGGW SGTDRAPPCEHGGIIRI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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