The biological activity was evidenced by the Resistin ability to prevent insulin-stimulated uptake of deoxy glucose in several cell lines at 10 ng/ml concentration corresponding to a Specific Activity of 100,000IU/mg.
Recombinant Human Resistin was lyophilized from a concentrated (1mg/ml) solution containing 0.03% NaHCO3.
Two-step procedure using size exclusion chromatography before and after refolding.
Greater than 98% as determined by SDS-PAGE.
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
The amino acid sequence of the Resistin Human Recombinant is homologous to the amino acid sequence of the Resistin Human without signal sequence.
Store lyophilized protein at -20oC. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4oC for a limited period of time; it does not show any change after two weeks at 4oC.
Cysteine-rich secreted protein FIZZ3, Adipose tissue-specific secretory factor, ADSF, C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein, Cysteine-rich secreted protein A12-alpha-like 2, RSTN, XCP1, RETN1, MGC126603, MGC126609.
