Request QuoteCatalog Number: xP516619DZHSize: 0.2-1mg

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Recombinant Replication protein 1a (ORF1a)

Recombinant Replication protein 1a (ORF1a) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516619DZHYeast1mgQuote
EP516619DZHE. coli1mgQuote
BP516619DZHBaculovirus200ugQuote
MP516619DZHMammalian Cell200ugQuote

Protein Information

SpeciesCucumber mosaic virus (strain NT9) (CMV)
UniProt IDO40976
Gene NameORFs:ORF1a
Protein NameReplication protein 1a Including the following 2 domains: ATP-dependent helicase
Region Expressed1-993
Expression Tag6xHis
Purity>90%
AA SequenceMATSSFNINELVASHGDKGLLATALVDKTAHEQLEEQLQHQRRGRKVYIRNVLGVKDSEV IRNRYGGKYDLHLTQQEFAPHGLAGALRLCETLDCLDSFPSSGLRQDLVLDFGGSWVTHY LRGHNVHCCSPCLGIRDKMRHAERLMNMRKIILNDPQQFDGRQPDFCTHPAADCKVQAHF AISIHGGYDMGFRGLCEAMNAHGTTILKGTMMFDGAMMFDDQGVIPELNCQWRKIRTAFS ETEDATPLSEKLNSTIFSHVRKFKTMVAFDFINESTMSYVHDWENIKSFLTDQTYSYRGM TYGIERCVIHAGIMTYKIIGVPGMCPPELIRHCIWFPSIKDYVGLKIPASQDLVEWKTVR ILTSTLRETEEIAMRCYNDKKAWMEQFKVILGVLSAKSSTIVINGMSMQSGERIDINDYH YIGFAILLHTKMKYEQLGKMYDMWNASYISKWFAALTRPLRVFFSSVVHALFPTLRPREE KEFLIKLSTFVTFNVECSFDGGEEWDVISSAAYVATQAVTDGKVLAAQKAEKLAEKLAQP VIEVSDRPEAPSSTPDDTADVCGKEQEVSELDSLSAQTRSPITRVAERATAMLEYAAYEK QLHDTTVSNLKRIWNMAGGDDKRNSLEGNLKFVFDTYFTVDPMVNIHFSTGRWMRPVPEG IVYSVGFNERGLGPKSDGELYIVNSECVICNSESLSTVTRSLQAPTGTISQVDGVAGCGK TTAIKSIFEPSTDMIVTANKKSAQDVRMALFKSSDSKEACTFVRTADSVLLNECPTVSRV LVDEVVLLHFGQLCAVMSKLKAVRAICFGDSEQIAFSSRDASFDMRFSKIIPDETSDADT TFRSPQDVVPLVRLMATKALPKGTHSKYTKWVSQSKVKRSVTSRAIASVTLVDLDSSRFY ITMTQADKASLISRAKEMNLPKTFWNERIKTVHESQGISEDHVTLVRLKSTKCDLFKQFS YCLVALTRHKVTFRYEHCGVLNGDLIAECIARA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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