$148.20Catalog Number: PYT555-10ugSize: 10ug

RBP4 Human, His

Retinol Binding Protein-4 Human Recombinant, His tag

RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa.
RBP4 is fused to N-terminus His tag which contains additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16-199.
The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques.

Accession

Q5VY30

Amino acid sequence

MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL.

Biological Activity

Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per M RBP4.

Formulation

RBP-4 His tag was filtered (0.4 um) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5.

Physical Appearance

White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Retinol binding protein-4 in sterile deionized water to a working volume of 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Source

Escherichia Coli.

Stability

Lyophilized Retinol binding protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Retinol binding protein-4 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Retinol Binding Protein 4 plasma, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP.

Quantity

$148.20

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