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Recombinant Queuine tRNA-ribosyltransferase (tgt)

Recombinant Queuine tRNA-ribosyltransferase (tgt) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP019146ESQYeast1mgQuote
EP019146ESQE. coli1mgQuote
BP019146ESQBaculovirus200ugQuote
MP019146ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DB26
Gene Nametgt; Locus:PC1_1019
Protein NameQueuine tRNA-ribosyltransferase
Region Expressed1-381
Expression Tag6xHis
Purity>90%
AA SequenceMKYELQTTDGRARRGRLIFERGVVETPAFMPVGTYGTVKGMTPEEVKDTGAQILLGNTFH LWLRPGQEIMKLHGDLHDFMQWHGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDSI FLSPEKSMEIQHDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRQRFDELENKN ALFGIIQGSVYEDLRDVSVKGLVDIGFDGYAVGGLAVGEPKEDMHRILEHVCPQIPEDKP RYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKDDVSPLDE HCDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRQAIEEGKLEHFVVD FYQRIGKPIPPLAEKDVAAGN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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