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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP708564Yeast1mgQuote
EP708564E. coli1mgQuote
BP708564Baculovirus200ugQuote
MP708564Mammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus kaustophilus (strain HTA426)
UniProt IDQ5L3K8
Gene NamerocA; Locus:GK0187
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMVQPYRHEPLTDFTVEANREAFLAALKKVESELGRDYPLVIGGERVMTEDKIISINPANK TEVVGRVAKANKELAERAMKTADEAFRTWSRTSPEARADILFRAAAIVRRRKHEFSAWLV KEAGKPWREADADTAEAIDFMEYYGRQMLKLKDGIPVESRPGETNRFFYIPLGVGVVISP WNFPFAIMAGTTVASLVTGNTVLLKPASATPVVAYKFVEVLEEAGLPAGVLNYIPGSGAE VGDYLVDHPRTRFISFTGSRDVGIRIYERAAKVHPGQIWLKRVIAEMGGKDAIVVDKEAD LELAAQSIVASAFGFSGQKCSACSRAIIVEDVYDQVLSRVVELTKQLNVGDPAEQATFMG PVIDQNAYNKIMEYIEIGKQEGRLMTGGEGDDAKGFFIQPTVFADVDPNARIMQEEIFGP VVAFAKARDFDHALEIANNTEYGLTGAVISRNRANLEKARHEFHVGNLYFNRGCTGAIVG YQPFGGFNMSGTDSKAGGPDYLILHMQAKTVSEMF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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