Request QuoteCatalog Number: xP511012GFJSize: 0.2-1mg

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Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA)

Recombinant 1-pyrroline-5-carboxylate dehydrogenase (rocA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP511012GFJYeast1mgQuote
EP511012GFJE. coli1mgQuote
BP511012GFJBaculovirus200ugQuote
MP511012GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D4J5
Gene NamerocA; Locus:GWCH70_0274
Protein Name1-pyrroline-5-carboxylate dehydrogenase
Region Expressed1-515
Expression Tag6xHis
Purity>90%
AA SequenceMVQPYKHEPLTDFTVEANRKAFEEALKKVEAELGKDYPLIIGGERVTTDEKIVSINPANK TEVIGRVSKANKELAEKAMKVADEAFKWWSKTKPEARADILFRAAAIVRRRKHEFSALMV KEAGKPWKEADADTAEAIDFMEYYARQMLKLKDGVPVESRPGETNRFFYIPLGVGVVISP WNFPFAIMAGTTVASLVTGNTVLLKPASATPVVAYKFAEVLEEAGLPAGVLNYIPGSGAE VGDYLVDHPRTRFISFTGSRDVGIRIYERAAKVHPGQIWLKRVIAEMGGKDGIVVDKEAD LELAAQSIVASAFGFSGQKCSACSRAIIVEDVYDQVLNRVVELTKQLKVGDPADQSTFMG PVIDQSAYNKIMEYIEIGKKEGRLMTGGEGDDSKGFFIQPTVFADVDPNARIMQEEIFGP VVAFTKAKDFDHALEIANNTEYGLTGAVISRNRANLEKAREEFHVGNLYFNRGCTGAIVG YQPFGGFNMSGTDSKAGGPDYLILHMQAKTVSEMF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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